Rapid protein quantification for His-tag protein expression screening
A new assay on the Amperia platform enables rapid protein quantification for His-tag protein expression screening, allowing teams to make informed decisions on construct scaling and purification. The assay provides quantitative data directly from unpurified samples, streamlining the protein expression and purification process.
Scientists have developed a new assay for rapid protein quantification. The Amperia platform uses a premix competition format to quantify His-tagged proteins in crude lysates. This enables teams to make decisions on construct scaling and purification earlier. A study with a global pharmaceutical company demonstrated the assay's effectiveness. Eight His-tagged protein constructs were expressed in E. coli and analyzed. The assay provided a clear ranking of construct expression levels. The same ranking was confirmed after purification. The assay streamlines the protein expression and purification process.
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